Journal: American Journal of Translational Research

Article Title: Periodontitis regulates renal impairment in obese mice via TGF-β/Smad pathway

doi:

Figure Lengend Snippet: Levels of TGF-β/Smad markers in periodontitis complicated with obesity. In vivo models of periodontitis complicated with obesity were established using silk ligatures with bacteria and high fat diet (n=10 for each group). A, B. Periodontitis complicated with obesity induced the upregulation of protein and mRNA levels of TGF-β in RTECs, while TGF-β siRNA downregulated TGF-β levels. C. Periodontitis complicated with obesity stimulated Smad2 phosphorylation (p-Smad2), and downregulating TGF-β inhibited p-Smad2 levels. D. Periodontitis complicated with obesity stimulated Smad3 phosphorylation (p-Smad3), and downregulating TGF-β inhibited p-Smad3 levels. E. Periodontitis complicated with obesity induced an increase in Smad7 levels, and downregulating TGF-β inhibited Smad7 levels. F. Western blots. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: Renal tubular epithelial cells (RTECs) were obtained from the American type culture collection (ATCC, USA) and cultivated in a complete culture medium (DMEM + 10% FBS containing 100 U/mL penicillin and streptomycin) in a 5% CO 2 , 37°C incubator.

Techniques: In Vivo, Bacteria, Phospho-proteomics, Western Blot

Journal: American Journal of Translational Research

Article Title: Periodontitis regulates renal impairment in obese mice via TGF-β/Smad pathway

doi:

Figure Lengend Snippet: Levels of TGF-β/Smad markers in periodontitis complicated with obesity in vitro. RTECs were treated with lipopolysaccharides from periodontal pathogens in a high fat environment to induce cell models of periodontitis and obesity. A, B. Periodontitis complicated with obesity induced the upregulation of protein and mRNA levels of TGF-β in RTECs, while TGF-β siRNA downregulated TGF-β levels. C. Periodontitis complicated with obesity stimulated Smad2 phosphorylation (p-Smad2), and downregulating TGF-β inhibited p-Smad2 levels. D. Periodontitis complicated with obesity stimulated Smad3 phosphorylation (p-Smad3), and downregulating TGF-β inhibited p-Smad3 levels. E. Periodontitis complicated with obesity induced an increase in Smad7 levels, and downregulating TGF-β inhibited smad7 levels. F. Western blots. *P<0.05; **P<0.01.

Article Snippet: Renal tubular epithelial cells (RTECs) were obtained from the American type culture collection (ATCC, USA) and cultivated in a complete culture medium (DMEM + 10% FBS containing 100 U/mL penicillin and streptomycin) in a 5% CO 2 , 37°C incubator.

Techniques: In Vitro, Phospho-proteomics, Western Blot

Journal: American Journal of Translational Research

Article Title: Periodontitis regulates renal impairment in obese mice via TGF-β/Smad pathway

doi:

Figure Lengend Snippet: Regulatory role of TGF-β/Smad pathway in the progression of periodontitis complicated with obesity in vitro. RTECs were treated with lipopolysaccharides from periodontal pathogens in a high fat environment to induce cell models of periodontitis and obesity. A. Periodontitis complicated with obesity promoted apoptosis in RTECs, and downregulating TGF-β inhibited the apoptosis. B-H. Periodontitis complicated with obesity elevated the protein and mRNA levels of inflammatory cytokines (TNF-α, IL-6 and MCP-1), and downregulating TGF-β inhibited those levels. I. Periodontitis complicated with obesity restricted the viability in RTECs, and downregulating TGF-β enhanced the viability. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: Renal tubular epithelial cells (RTECs) were obtained from the American type culture collection (ATCC, USA) and cultivated in a complete culture medium (DMEM + 10% FBS containing 100 U/mL penicillin and streptomycin) in a 5% CO 2 , 37°C incubator.

Techniques: In Vitro

Journal: American Journal of Translational Research

Article Title: Periodontitis regulates renal impairment in obese mice via TGF-β/Smad pathway

doi:

Figure Lengend Snippet: Measurement of renal pathological changes in each group (n=10) by HE staining. A. Renal sections were examined pathologically under 100× magnification; scale. B. Renal sections were examined pathologically under 400× magnification; scale. In control group, structures of renal tubules and glomeruli are clear, with orderly arranged RTECs. While periodontitis complicated with obesity led to an increase in glomerular volume, unclear glomerular structure, smaller Bowman’s capsule and glomerulosclerosis; Moreover, RTECs swell into vacuoles and show dilatation of the lumen. After the TGF-β/Smad pathway was downregulated, the kidney structure improved evidently, the glomerular volume decreased, Bowman’s lumen and glomerular structure were clear, and RTECs recovered their orderly arrangement.

Article Snippet: Renal tubular epithelial cells (RTECs) were obtained from the American type culture collection (ATCC, USA) and cultivated in a complete culture medium (DMEM + 10% FBS containing 100 U/mL penicillin and streptomycin) in a 5% CO 2 , 37°C incubator.

Techniques: Staining, Control

Journal: International Journal of Molecular Sciences

Article Title: Urinary Neuropilin-1: A Predictive Biomarker for Renal Outcome in Lupus Nephritis

doi: 10.3390/ijms20184601

Figure Lengend Snippet: NRP-1 induced proliferation and migration in HRECs. ( a ) Expression levels of NRP-1 after non-stimulation (NS), interleukin-1 alpha (IL1-a) and vascular endothelial growth factor (VEGF) stimulation in primary human cells: renal mesangial cells (HRMCs), renal endothelial cells (HRECs), T cells and renal tubular epithelial cells (RTECs). One-way ANOVA (correction Bonferroni). *** p < 0.0001. ( b ) Proliferation assay in inhibited NRP-1 and control with VEGF stimulation or with non-stimulation conditions (NS). Student’s t -test. *** p < 0.0001 ( c ) A straight scratch was performed to simulate a wound. After that, the distance between wound edges was determinate overtime and in each condition. Migration rate was expressed as percentage of confluence index (CI) between the performed scratch and calculated using the distance between wound edges. Photographs were captured by a phase contrast microscope. Bars, 2 mm ( d ) Analysis of mRNA expression levels between inhibited NRP-1 HRECs in VEGF or NS conditions. Fold change was calculated over their transfection control. Student’s t-test. *** p < 0.0005 ( e ) Immunofluorescence of VEGFR2 (green) and NRP1 (red) in HRECs after transfection and stimulation. Bars, 50 µm. DAPI staining was used to label cell nuclei. Student’s t -test. * p < 0.05. HRECs: Human Renal Endothelial Cells.

Article Snippet: Primary Human Renal Endothelial Cells (HRECs), Primary Human Renal Glomerular Mesangial cells (HRMCs) and Primary Human Renal Tubular Epithelial cells (RTECs) were purchased and cultured in the recommended media provided by the manufacturer (Innoprot, Derio, Bizkaia, Spain).

Techniques: Migration, Expressing, Proliferation Assay, Microscopy, Transfection, Immunofluorescence, Staining

Journal: Frontiers in Microbiology

Article Title: ChromID ® CARBA Agar Fails to Detect Carbapenem-Resistant Enterobacteriaceae With Slightly Reduced Susceptibility to Carbapenems

doi: 10.3389/fmicb.2020.01678

Figure Lengend Snippet: Strains and species used in this study with their resistance determinants (threefold determined MIC values, variations are indicated with a “/” between the values ).

Article Snippet: The quality control isolate AR-1, a CTX-M-1-producing E. coli with a low phenotypical resistance against ertapenem ( and ), and the quality control strain ATCC 25922 did not grow on ChromID ® CARBA plates at any time ( ).

Techniques: Bla VIM Assay